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S. typhoid/S.Para typhi Ag Rapid Test Chipangizo

S. typhoid/S.Para typhi Ag Rapid Test Chipangizo

Matenda a S. typhoid/S.Para typhi Ag Rapid Test Device (Nyenyewe) ndi lateral flow immunoassay kwa kuzindikira munthawi yomweyo ndi kusiyanitsa Salmonella typhi ndi Salmonella P. Typhoid mu ndowe.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mfundo yofunika

Matenda a S. typhoid/S.Para typhi Ag Rapid Test Device (Nyenyewe) ndi lateral flow chromatographic immunoassay.Kaseti yoyesera ili ndi: 1) padi yamtundu wa burgundy conjugate yokhala ndi S.typhoid/S.Antibody ya Para typhi yolumikizidwa ndi golidi wa colloid, 2) mzere wa membrane wa nitrocellulose wokhala ndi magulu awiri oyesera (magulu a S. typhoid/S. Para typhi) ndi gulu lowongolera (C band).Gulu la S. typhoid limakutidwa kale ndi monoclonal anti- S. typhoid kuti lizindikire S. typhoid Ag, S. Para typhi band limakutidwa kale ndi ma reagents kuti azindikire S. Para typhi Ag, ndi C band. imakutidwa kale ndi anti mouse IgG.

Pamene chiŵerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha chitsanzo cha kaseti, chitsanzo choyesera chimasuntha ndi capillary action kudutsa kaseti yoyesera.S. typhoid Ag ngati alipo mu chitsanzo cha odwala adzamanga ku S. typhoid Ab conjugates.The immunocomplex ndiye anagwidwa pa nembanemba ndi pre- TACHIMATA S. typhoid antibody, kupanga burgundy wachikuda S. typhoid gulu, kusonyeza S. typhoid zabwino mayeso zotsatira.

S.Para typhi Ag ngati alipo mu chitsanzo cha wodwala adzamanga kwa S. Para typhi Ab conjugates.The immunocomplex ndiye anagwidwa ndi chisanadze TACHIMATA S. Para typhi Ab pa nembanemba, kupanga burgundy wachikuda S. Para typhi Ab gulu, kusonyeza S. Para typhi Ab zabwino mayeso zotsatira.Kusakhalapo kwa magulu aliwonse oyeserera kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex ya mbuzi anti Mouse IgG/Mouse IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse oyesa.

Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.

Njira Yoyesera

Bweretsani zoyeserera, zoyeserera, zotchingira ndi/kapena zowongolera ku kutentha kwachipinda (15-30°C) musanagwiritse ntchito.

1. Kutolereni zitsanzo ndi kuchiza:
1) Tsegulani ndi kuchotsa dilution chubu applicator.Samalani kuti musatayike kapena kumwaza madzi kuchokera mu chubu.Sonkhanitsani zitsanzo polowetsa ndodo yolemberamo osachepera
3 malo osiyanasiyana a ndowe.
2) Bwezerani chogwiritsira ntchito mu chubu ndikupukuta kapu mwamphamvu.Samalani kuti musathyole nsonga ya chubu cha dilution.
3) Gwirani mwamphamvu chubu chosonkhanitsira chitsanzo kuti musakanize chitsanzocho ndi bafa yochotsa.Zitsanzo zokonzedwa mu chubu chotolera zitsanzo zitha kusungidwa kwa miyezi 6 pa -20°C ngati sizinayesedwe mkati mwa ola limodzi mutakonzekera.

2. Kuyesedwa
1) Chotsani mayesowo m’thumba lake lomatapo, ndikuchiyika pamalo oyera, osalala.Lembani mayesowo ndi chizindikiritso cha wodwala kapena wowongolera.Kuti mupeze zotsatira zabwino, kuyesako kuyenera kuchitidwa mkati mwa ola limodzi.
2) Pogwiritsa ntchito pepala la minofu, chotsani nsonga ya chubu chotsitsa.Gwirani chubu molunjika ndikugawira madontho atatu amadzimadzi mu chitsime cha chitsanzo (S) cha chipangizo choyesera.
Pewani kukokera thovu la mpweya pachitsanzo chabwino (S), ndipo musagwetse njira iliyonse pawindo lowonera.
Pamene mayesero ayamba kugwira ntchito, mudzawona mtundu ukusuntha pa nembanemba.
3. Dikirani kuti magulu achikuda awonekere.Zotsatira zake ziyenera kuwerengedwa kwa mphindi 10.Osamasulira zotsatira pakatha mphindi 20.


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