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Nucleic acid kuzindikira kwa fluorescence kuchuluka kwa Ct mtengo - chizindikiro cha chidani chachikondi

Nucleic acid kuzindikira kwa fluorescence kuchuluka kwa Ct mtengo - chizindikiro cha chidani chachikondi

"New Coronary Virus Pneumonia Diagnosis and Treatment Programme (Trial Ninth Edition)" imagwiritsa ntchito nucleic acid discovery Ct value ≥35 monga maziko ofunikira otulutsira kasamalidwe ka kudzipatula kapena kutulutsa.Ndiye, mtengo wa Ct wa zida zodziwira za nucleic acid umayimira chiyani?Kodi ma Ct amitundu yosiyanasiyana amafanana?Kodi kutsika kwa mtengo wa Ct, kumapangitsa kuti zidazo ziziyenda bwino?
Mtengo wa Ct (Threshold Cycle, Ct) ndi chiwerengero cha mikombero ya PCR pamene mphamvu ya siginecha ya fulorosenti ya nthawi yeniyeni ya fluorescence idutsa malire okhazikitsidwa.Zida zozindikirira za Nucleic acid zimatenga korona watsopano monga chitsanzo.Pakuchita chimodzimodzi kwa zida zomwezo kuti muwone zitsanzo ziwiri zomwe zili ndi kachilomboka, A ndi B, kukula kwa mtengo wa Ct kumayimira, pamlingo wina, chiwerengero cha makope a jini, ndiko kuti, kuchuluka kwa ma virus.Kutsika kwa Ct ya chitsanzo B, kumapangitsa kuchuluka kwa ma virus.Mtengo wa Ct umasiyana mosagwirizana ndi kuchuluka kwa ma virus komanso kusakhudzidwa kwake.Ubwino ndi kuipa kwa zida zosiyana siyana za nthawi yeniyeni ya fluorescence sizingaganizidwe kokha ndi kukula kwa mtengo wa Ct, chifukwa ma Ct amtundu wa zida zosiyana sangafanane.Kuunikira kwa magwiridwe antchito a zida kuyenera kuyezedwa kuchokera kuzinthu zokhuza (kuchepera kwa malire), kutsimikizika, kulondola, kukhazikika, ndi kugwiritsa ntchito zowunikira.Aliyense wopanga reagent ali ndi mitundu yake khumi ndi isanu ndi itatu ya masewera a karati, ndipo ali ndi mphamvu zawo zamatsenga zomwe akugwiritsa ntchito, kotero sindifotokoza mwatsatanetsatane apa.
Pazida zodziwikiratu zenizeni zenizeni za PCR (QPCR) zowunikira, kupeza kwa Ct values ​​ndikugwiritsa ntchito kwawo ponseponse pakuwunika kwa tizilombo toyambitsa matenda ndizodziwika kwa aliyense.Ndi chitukuko cha mafakitale ndi kupita patsogolo kwaukadaulo, zida za QPCR ndi ma reagents akumana ndi mwayi ndi zovuta.Yiou Think Tank (2021 Research Report on China's Genetic Testing Industry: Technology) anaunika kuti makampani ambiri m'makampani a QPCR mdziko langa akukumana ndi mavuto omwe amabwera chifukwa chaukadaulo.Poyankha mavutowa, mayankhowo amagawidwa m'mitundu iwiri: kufulumizitsa kukonza kwaukadaulo ndikugwiritsa ntchito matekinoloje atsopano.
POCT ya QPCR yozindikira ma cell ndi imodzi mwamagawo a chitukuko chamakampani, ndipo kuwongolera magwiridwe antchito ndichinthu chofunikira kwambiri kuti ifulumizitse kugwiritsa ntchito ukadaulo.Pazida za POCT zokhala ndi chitsanzo chimodzi cha QPCR, momwe mungakwaniritsire mtengo wa Ct pagawo lofunikira pakuchita kamodzi mu R&D?Zida zomwezo, machitidwe omwewo, ndi ndende yofananira ya template, mongoyerekeza kukulira kwa mtengo wa Ct pakuchita kumodzi, kumachepetsa mphamvu ya enzyme muzochita.
Pakuwongolera magwiridwe antchito a enzyme ndikuwongolera mtengo wa Ct, 40 PCR cycle fluorescence quantitative amplification curve ikhoza kuwunikidwa poyamba.Mapiritsi okulitsa pachithunzi pansipa agawidwa mu gawo lakumbuyo la fluorescence, gawo lokulitsa mawu, gawo la mzere, ndi gawo lamapiri.Kuti mukwaniritse maziko otsika a fluorescence, momwe mungathere kuti musiyanitse ndi mtengo wa fluorescence wa kukulitsa kwachidziwitso koyambirira, ndikofunikira kuyesa kuchokera ku zonyamulira zonyamula, kuwunika koyambira, ndi kachitidwe ka buffer.Nthawi yokulitsa ma exponential ndiye machitidwe achindunji a zochitika za ma enzyme, komanso ndi umboni wa kulumikizana kwabwino kwa zida za POCT, zonyamula ma reaction ndi ma reagents.Panthawiyi, kuwongolera kutentha kwa zida, kupeza ndi kusanthula ma sign optical, carrier biocompatibility, ndi machitidwe a reagents amayenera kusinthidwa ndikuyesedwa m'mbali zonse.Potsirizira pake, kukulitsa konseko kumatsirizidwa, ndipo zotsatira ziyenera kuperekedwa ndi ndondomeko yowonongeka ya deta kuti ipeze mtengo wokwanira wa Ct.
CT line
Gawo laling'ono pakukhathamiritsa kwa mtengo wa Ct ndi masitepe osawerengeka a ogwira ntchito ku R&D.Mu nkhawa ndi chisangalalo cha usana ndi usiku, nthawi zonse "mapiri ndi mitsinje siziwoneka, misondodzi imakhala yakuda ndi maluwa owala ndipo pali mudzi wina", zomwe zimatipatsa kulimba mtima ndi chilakolako chopita patsogolo. kachiwiri.


Nthawi yotumiza: Sep-29-2022