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Cytomegalovirus One Step CMV IgG/IgM Rapid Test Package Insert

Cytomegalovirus One Step CMV IgG/IgM Rapid Test Package Insert

The One Step CMV IgG/IgM Rapid Test Device ndi mayeso othamanga othamanga omwe amapangidwa kuti azindikire kuchuluka kwa ma antibodies a IgG ndi IgM ku Cytomegalovirus (CMV) m'magazi amunthu Onse, seramu kapena plasma.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Mfundo yofunika

Ndi chromatographic immunoassay yofulumira yozindikira ma antibodies (IgG ndi IgM) kupita ku CMV mumagazi Athunthu, Seramu kapena Plasma.Chiyeso chilichonse chimakhala ndi: 1) pad yamtundu wa burgundy yokhala ndi ma antigen a CMV recombinant envelopu yolumikizidwa ndi golide wa Colloid (CMV conjugates) ndi ma conjugates a kalulu IgG-gold,2) mzere wa nitrocellulose wokhala ndi magulu awiri oyesera (T1 ndi T2 band) ndi gulu lowongolera (C band).Gulu la T1 limakutidwa kale ndi anti-CMV kuti lizindikire IgM anti-CMV, gulu la T2 limakutidwa ndi anti-CMV kuti lizindikire IgG anti-CMV, ndipo C band idakutidwa kale ndi anti rabbit IgG.Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha chitsanzo cha kaseti yoyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.IgG anti-CMV, ngati ilipo pachitsanzocho, imamangiriza ku ma conjugates a CMV.Immunocomplex imatengedwa ndi reagent yomwe idakutidwa kale pa bandi ya T2, ndikupanga gulu la T2 lamtundu wa burgundy, kuwonetsa zotsatira za mayeso a CMV IgG ndikuwonetsa matenda aposachedwa kapena obwereza.IgM anti-CMV ngati ilipo mu chitsanzo idzamangiriza ku ma conjugates a CMV.The immunocomplex kenako amagwidwa ndi reagent yokutidwa pa T1 band, kupanga burgundy mtundu T1 gulu, kusonyeza CMV IgM zabwino mayeso ndi kusonyeza matenda atsopano.Kusakhalapo kwa T band (T1 ndi T2) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chikuyenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse a T.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.

Ndondomeko

1. Bweretsani thumbalo kuti lizizizira bwino musanatsegule.Chotsani chipangizo choyesera m'thumba lomata ndikuchigwiritsa ntchito posachedwa.

2. Ikani chipangizo choyesera pamalo oyera komanso osakanikirana.

3. Gwirani chotsitsa chotsika ndikusamutsa dontho limodzi la Plasma/seramu (pafupifupi 10μl) kapena madontho awiri amagazi athunthu (pafupifupi 20μl) kuchitsime chachitsulo (S) cha chipangizo choyesera, kenako onjezerani madontho awiri a buffer (pafupifupi 20μl). 80μl) ndikuyamba chowerengera.Onani chithunzi pansipa.

4. Dikirani kuti mizere yachikuda iwonekere.Werengani zotsatira pa mphindi khumi ndi zisanu.Osamasulira zotsatira pakatha mphindi 20.

Ndemanga:
Kugwiritsa ntchito kuchuluka kokwanira kwachitsanzo ndikofunikira kuti mupeze zotsatira zolondola.Ngati kusamuka (kunyowetsa kwa nembanemba) sikukuwoneka pazenera loyesa pakatha mphindi imodzi, onjezani dontho lina la buffer pachitsanzocho.

Kulondola:
Zotsatira zinawonetsa> 99% kulondola kwathunthu kwa hCG One Step Pregnancy Combo Test Device poyerekeza ndi mayeso ena a mkodzo a hCG.

Zotsatira

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IgM POSITIVE: * Mzere wachikuda mu gawo la mzere wowongolera (C) umawonekera ndipo mzere wachikuda umapezeka mugawo loyesa 1 (T1).Zotsatira zake zikuwonetsa kukhalapo kwa ma antibodies a CMV enieni a IgM.

IgG POSITIVE: * Mzere wachikuda mu gawo la mzere wowongolera (C) umawonekera ndipo mzere wamitundu umawoneka mugawo la mayeso 2 (T2).Zotsatira zake zikuwonetsa kukhalapo kwa ma antibodies a CMV enieni a IgG.

IgG NDI IgM POSITIVE: * Mzere wachikuda mu gawo la mzere wowongolera (C) ukuwonekera ndipo mizere iwiri yamitundu ikuyenera kuwonekera pamagawo oyesa 1 ndi 2 (T1 ndi T2).Kuchuluka kwamtundu wa mizere sikuyenera kufanana.Zotsatira zake zikuwonetsa kuti kukhalapo kwa ma antibodies a CMV enieni a IgG ndi IgM.

*ZOYENERA: Kuchuluka kwa mtundu mumzere woyesera (T1 ndi/kapena T2) kumasiyana malinga ndi kuchuluka kwa ma CMV achitetezo pachitsanzocho.Chifukwa chake, mtundu uliwonse wamtundu pamagawo oyesera (T1 ndi/kapena T2) uyenera kuwonedwa ngati wabwino.

ZOTSATIRA ZAKE ZOSAVUTA:
Mzere wachikuda mu gawo la mzere wowongolera (C) ukuwonekera.Palibe mzere womwe umapezeka m'magawo oyesa 1 kapena 2 (T1 kapena T2).

ZOTSATIRA ZAKE:

Mzere wowongolera ukulephera kuwonekera.Kusakwanira kwa kuchuluka kwa zitsanzo kapena njira zolakwika ndizo zifukwa zomwe zimalepheretsa mzere wowongolera.Unikaninso ndondomekoyi ndikubwereza kuyesanso ndi chipangizo chatsopano choyesera.Vuto likapitilira, siyani kugwiritsa ntchito zida zoyeserera nthawi yomweyo ndipo funsani wofalitsa wapafupipafupi.

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